Enzymes Cambridge IGCSE Biology revision
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In plain words
The chemical reactions that keep you alive would be far too slow on their own. Enzymes are proteins that speed them up. Each one has a pocket shaped to fit one particular molecule, like a lock that takes only one key, so each enzyme does only one job.
Three things to know
- Enzymes are proteins that act as biological catalysts: they speed up reactions without being used up.
- The substrate fits into the enzyme's active site because their shapes are complementary. That is why each enzyme works on only one substrate.
- An enzyme works fastest at its optimum temperature and pH. Too hot, or the wrong pH, and the active site changes shape so the substrate no longer fits: the enzyme is denatured.
Tips and tricks
- Enzymes are denatured, not killed. They were never alive.
- Low temperature only slows an enzyme down (fewer collisions). High temperature denatures it, and that can't be undone.
It lands in your notebook with its questions as flashcards.
Enzymes: 5 questions and answers
These are the quiz’s questions. Do the quiz first, then come back here for the ones that got you.
What kind of molecule is an enzyme?
All enzymes are proteins.
What is the part of an enzyme where the substrate fits?
Its shape is complementary to the substrate.
What happens to an enzyme at a very high temperature?
The active site changes shape and the substrate can no longer fit.
Why does each enzyme work on only one substrate?
This is called specificity.
What happens to an enzyme at the end of a reaction?
That is what makes it a catalyst.
Quiz
5 questions
Tap an answer and you’ll see straight away whether it’s right, and why.
Worksheet
3 questions, 7 marks. Write your answers on paper, then check them.
Enzymes
Cambridge IGCSE Biology 0610 · 7 marks · papermunch.org
Name ______________________________ Date ______________
State what is meant by an enzyme.[2]
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A protein that acts as a biological catalyst, speeding up a reaction without being changed.
Explain why an enzyme stops working when it is heated to 70 °C.[3]
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The high temperature changes the shape of the active site (the enzyme is denatured), so the substrate no longer fits and no reaction happens.
Explain why amylase breaks down starch but not protein.[2]
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The active site of amylase has a shape that is complementary to starch only. Protein molecules do not fit.
Answers: Enzymes
- 1. A protein that acts as a biological catalyst, speeding up a reaction without being changed.
- 2. The high temperature changes the shape of the active site (the enzyme is denatured), so the substrate no longer fits and no reaction happens.
- 3. The active site of amylase has a shape that is complementary to starch only. Protein molecules do not fit.



